Author: Herr Dr. Amoneit
Last saved by: Saritas, Sevim

New UHPLC-QqQ-MS/MS Method for Quantitative and Qualitative Determination of 10 Phytoprostanes in Foodstuffs of Commercial Olive and Sunflower Oils
Jacinta Collado-González^1, Sonia Medina^1, Thierry Durand^2, Alexandre Guy^2, Jean-Marie Galano^2, Arturo Torrecillas^3, Federico Ferreres^1, Angel Gil-Izquierdo^1.
^1Department of Food Science and Technology. CEBAS-CSIC. Campus de Espinardo. P.O. Box 164, 30100 Espinardo, Murcia, Spain.
^2Institut des Biomolécules Max Mousseron, UMR 5247 CNRS-Universités Montpellier 1 & Montpellier 2 - ENSCM, Montpellier, France
^3 Deparment of Irrigation. CEBAS-CSIC. Campus de Espinardo-25. Murcia, Spain

Fairly recently, some researchers have studied the relationship of oxidative stress and the content of compounds called phytoprostanes (PhytoPs) in plants. In this work, we propose a new quick and accurate analytical method, which is more sensitive and more selective than GC-MS methods and is able to identify 10 PhytoPs, including their regioisomers and mixture of enantiomers in a single injection in olive and refined sunflower oils. The recovery provided high extraction efficiencies ranging from 102.90 to 140.64 % using Strata-XAW cartridge. The intra-day variations for all target compounds ranged from 2.07 to 14.35% and the accuracies varied from 81.14 to 119.56%. The inter-day variations for the same compounds were from 0.01 to 14.48% and the accuracies from 80.72 to 119.56%. The LOD values of our method ranged from 0.29 to 2.56 ng/mL and the LOQs from 0.59 to 5.12 ng/mL. Results obtained by this new method reflect that refined sunflower presented more series of PhytoPs and a 20 and 8 fold higher quantity in two types of olive oil: Extra virgin olive oil and olive oil, (containing half virgin extra olive oil and half refined olive oil). The manufacture process could be the key for the different PhytoP production since most of the plant oils are subjected to a refining treatment.