Molecular Species Analysis of Triglycerides in different Plant Oils by LC-APCI-MS with Silver Ion Columns in Series
Wei Tingting, Yang Hong, Jiang Yuanrong, Wilmar(Shanghai) Biotechnology Research & Development Center Co.,Ltd.
Shanghai, China 

A high performance liquid chromatography with Atmospheric Pressure Chemical Ionization mass spectrometry (HPLC-APCI-MS) method utilizing silver ion columns in series is developed for separation of triglycerides in 12 kinds of different plant oils, such as soybean oil(SBO), maize oil(MZO), rapeseed oil(RSO), high erucic rapeseed oil(HERSO), palm oil(PO), peanut oil(GNO), rice bran oil(RBO), tea seed oil(TSO), Virgin olive oil(VOL), sesame oil(SSO), flaxseed oil（FSO）and sunflower seed oil(SFO). Triglycerides, which can be identified by retention time and mass fragments, are summarized. And the triglyceride contents are measured by percentage of peak area in different oils. The paper also discussed the situation and law of triglycerides eluted by silver ion columns. The result shows that there are some specific triglycerides in different plant oils, furthermore, contents of some nonspecific triglycerides are dissimilar. It helps us to identify the adulterated oils and calculate contents of several oils in blend oil based on statistics.