Effect of Refining Practice on Tocopherols Content of Sunflower Oil
Abdul Rauf Khaskheli1,4,  Saba Naz2,3,4, S.T.H.  Sherazi2, Sirajuddin2
1Shaheed Mohtarma Benazir Bhutto Medical University Larkana, Pakistan
2University of Sindh, National Center of Excellence in Analytical Chemistry, Jamshoro, Pakistan
3University of Sindh, Dr.M.A.Kazi Institute of Chemistry, Jamshoro, Pakistan
4Selcuk University, Fen-Edebiyat Fakültesi, Kimya Bölümü, Kampus,42075 Konya, Turkey

The changes of tocopherols were investigated during different sunﬂower oil refining stages by reverse-phase high-performance liquid chromatography. The results revealed that the levels of tocopherol content were decreased during the neutralization, bleaching, and deodorization processes. The overall loss of total tocopherols during these stages was found to be 37.9%, although the general reduction trend of α, (β+γ) and δ, tocopherols are very similar during neutralization (35.3%), bleaching (38.3%), and deodorization (37.8%). However, in contrast to the neutralizing and deodorizing stages, the bleaching process caused a relatively less reduction for individual tocopherol contents. Deodorizer distillates were also analyzed and were found to be rich with tocopherols content (29,348.24 µg/ml).