Synthesis of Phosphatidylcholine with Conjugated Linolenic Acids in Lipase-catalyzed Transesterification Process
Anna Chojnacka, Witold Gładkowski, Anna Gliszczyńska, Czesław Wawrzeńczyk 
Wroclaw Environmental and Life Sciences, Department of Chemistry, Wrocław, Poland

	Conjugated linolenic acids (CLnA) are the positional isomers of linolenic acids (C18:3) which contain conjugated double bonds and they have shown interesting biological effects such as: anticarcinogenic, prevention and treatment of inflammation [1] improving immune function and lipid metabolism [2], decrease body fat content, hipolipidemic and antioxidant [3]. Phospholipids have also shown important biological functions and health benefits such as reducing blood cholesterol and triglycerides [4]. Structured phospholipids with conjugated linolenic acid may be desirable for the development of “nutraceutical lipids” which may improve health and prevent or treat disease.
	Our research is focused on the synthesis of structured phospholipids in transesterification process of egg-yolk phosphatidylcholine (PC) with pomegranate seed oil which contain about 77% of punic acids (C 18:3, 9cis, 11trans, 13cis). The reactions were carried out in hexane, at 50 oC, with different molar ratio of oil/PC and enzyme loading. The maximum incorporation of CLnA from pomegranate oil into PC was about 29% after 72 h of reaction carried out with 20% Novozym 435 loading and 2:1 oil/PC molar ratio.
Acknowledgements: 
The project was financed by the National Science Centre granted  on the basis of the decision no DEC-2012/05/B/NZ9/03358.

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